Protein Expression

With our proven track records in G protein-coupled receptor and other protein expression and analysis, we offer following services:

Baculovirus Expression and Analysis

  • Insect cell transfection with bacmid (pFASTbac based vector)
  • Small scale expression: 4 ml, 40 ml, 250 ml
  • Large scale expression: 1L, 2L, 3L
  • Baculovirus generation and titer
Baculovirus protein expression is conducted using adapted Sf9 (Spodoptera frugiperda) suspension cells, which will be transfected with a CMV-based expression plasmid at the log phase. The expressed protein will be labeled with specific antibody to enable expression measurement using Guava® easyCyte Flow Cytometers, where it will estimate the expression levels of the target protein both on cell membrane and inside of cells.

Mammalian Expression and Analysis

  • Transfection with purified DNA
  • Small Scale expression: 2.5 ml, 30 ml, 200 ml
  • Large scale expression: 1L, 2L
Mammalian protein expression is conducted using adapted suspensions of HEK 293F cells, which will be transfected with a CMV-based expression plasmid at the log phase. The expressed protein will be labeled with specific antibody to enable expression measurement using Guava® easyCyte Flow Cytometers, where it will estimate the expression levels of the target protein both on cell membrane and inside of cells.

Additional Info:

Our services in baculovirus and mammalian expressions are comprehensive and can be combined with other services at the Structural Biology Center. The protein expression can be combined with protein purification, biochemical assays, protein crystallization and structure determination of the expressed protein. Our team members are experts in their fields, our services are competitive in pricing and our well-established protocols and facilities makes for a rapid turnaround time. All of our data are databased, archived and easily available in spreadsheet format.
Please feel free to call us at (213) 740-8634 for additional information or questions.

Please see the rates for protein expression at https://bridge.usc.edu/sbc/spexpr.html