Vapor Diffusion Crystallization >>

LCP Crystallization >>

The start point at SBC will be having pure and functional protein that can be run on crystallization trials. However if you have problem producing your protein, our experts will provide you with valuable information to solve your problem.

Once you have your protein ready, we will set up initial crystallization screens. When a hit is observed, the crystallization condition will be optimized for higher quality crystals, which in a later stage will be prepared in several cryo conditions (soluble proteins) for being frozen in liquid nitrogen.

The frozen crystals will be exposed for X-Ray radiation which will result in a diffraction. The collected diffraction data will be processed and will be used for structural determination using Molecular replacement or other phasing strategies like MAD, SAD, SIR, and SIRAS.